Preparation of single-stranded PCR merchandise for electrospray ionization mass spectrometry utilizing the DNA restore enzyme lambda exonuclease.
Electrospray ionization mass spectrometry (ESI-MS) has been utilized to acquire correct mass measurements of intact PCR merchandise; nonetheless, single-stranded PCR merchandise are essential to detect sequence modifications comparable to base substitutions, additions or deletions. The areas of those modifications can subsequently be decided utilizing extra phases of mass spectrometry.
- The recombinant enzyme lambda exonuclease selectively digests one strand of a DNA duplex from a 5′ phosphorylated finish leaving the complementary strand intact. Utilizing this speedy enzymatic step, we had been capable of produce single-stranded PCR merchandise by digestion of an intact PCR product derived from the Human Tyrosine Hydroxylase (HUMTHO1) gene, which incorporates a tetrameric repeating motif.
- The non-template directed 3′ adenylation frequent when utilizing Taq polymerase resulted in three distinct species (blunt-ended, mono-adenylated and di-adenylated), which added complexity to the spectrum of the double-stranded product.
- The info from the single-stranded merchandise reveals that one strand is preferentially adenylated over the opposite, which can’t be decided from the mass spectrum of the double-stranded PCR product alone. The ESI-FTICR (Fourier rework ion cyclotron resonance) mass spectra of the lambda exonuclease handled PCR merchandise exhibited lower than anticipated signal-to-noise (S/N) ratios.
- That is attributed to inaccurate focus calculations attributable to remaining double-stranded PCR product amplified with unphosphorylated primers, and to matrix results contributed by the lambda exonuclease response buffer.
- To additional take a look at this speculation, we investigated and decided the limit of detection to be 0.27 microM utilizing customary curve statistics for single acquisitions of an artificial 75-mer. The concentrations of the noncoding and coding strands produced by lambda exonuclease digestion had been calculated to be 0.29 and 0.37 microM, respectively, bearing in mind the presence of double-stranded product.
- The merchandise had been electrosprayed from concentrations on the restrict of detection requiring the averaging of 5-10 acquisitions to provide a enough S/N ratio, indicating that product focus, base composition and matrix results play a mixed, vital position in detection of lambda exonuclease handled PCR merchandise.
- Though extra work shall be required to additional exploit this technique, lambda exonuclease clearly gives mass spectrometrists with a technique to generate single-stranded PCR merchandise.
SIV p55, Recombinant (Simian Immunodeficiency Virus) |
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MBS636423-5x001mg | MyBiosource | 5x0.01mg | EUR 2210 |
SIV gp130, mac251 (Simian Immunodeficiency Virus) |
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MBS604242-01mg | MyBiosource | 0.1mg | EUR 795 |
SIV gp130, mac251 (Simian Immunodeficiency Virus) |
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MBS604242-5x01mg | MyBiosource | 5x0.1mg | EUR 3435 |
SIV nef, mac251, Recombinant (Simian Immunodeficiency Virus) |
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MBS637605-01mg | MyBiosource | 0.1mg | EUR 1210 |
SIV nef, mac251, Recombinant (Simian Immunodeficiency Virus) |
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MBS637605-5x01mg | MyBiosource | 5x0.1mg | EUR 5225 |
SIV gp130, mac251, Recombinant (Simian Immunodeficiency Virus) |
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MBS635667-01mg | MyBiosource | 0.1mg | EUR 1210 |
SIV gp130, mac251, Recombinant (Simian Immunodeficiency Virus) |
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MBS635667-5x01mg | MyBiosource | 5x0.1mg | EUR 5225 |
Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) REV Polyclonal Antibody |
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MBS7152686-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) VPR Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) NEF Polyclonal Antibody |
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MBS7153268-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) GAG Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) TAT Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) ENV Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) VIF Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K78)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) REV Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) REV Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K78)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) NEF Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K78)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VPR Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K78)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) ENV Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K78)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) TAT Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VPX Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VPR Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) NEF Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) GAG Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VIF Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) TAT Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate STM)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) GAG Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) ENV Polyclonal Antibody |
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MBS7162159-INQUIRE | MyBiosource | INQUIRE | Ask for price |
SIV (Simian Immunodeficiency Virus) gp160 Fragment, aa 171-190-Cys |
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MBS658378-1mg | MyBiosource | 1mg | EUR 610 |
SIV (Simian Immunodeficiency Virus) gp160 Fragment, aa 171-190-Cys |
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MBS658378-5x1mg | MyBiosource | 5x1mg | EUR 2600 |
Rabbit anti-Simian immunodeficiency virus (isolate GB1)(SIV-mnd)(Simian immunodeficiency virus mandrill) gag-pol Polyclonal Antibody |
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MBS7152687-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate 1A11)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) NEF Polyclonal Antibody |
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MBS7154036-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm251)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) TAT Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate Mm251)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) REV Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate Mm251)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) ENV Polyclonal Antibody |
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MBS7164805-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate K6W)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) gag-pol Polyclonal Antibody |
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MBS7164533-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) TAT Polyclonal Antibody |
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MBS7164540-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VPX Polyclonal Antibody |
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MBS7164542-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VPR Polyclonal Antibody |
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MBS7164552-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) NEF Polyclonal Antibody |
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MBS7162149-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) REV Polyclonal Antibody |
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MBS7162155-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) ENV Polyclonal Antibody |
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MBS7162160-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) GAG Polyclonal Antibody |
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MBS7162164-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) VIF Polyclonal Antibody |
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MBS7162169-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Simian immunodeficiency virus (SIV) (isolate 216.94.A2) gp120 Protein (His Tag) |
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MBS8118796-01mg | MyBiosource | 0.1mg | EUR 450 |
Simian immunodeficiency virus (SIV) (isolate 216.94.A2) gp120 Protein (His Tag) |
|||
MBS8118796-5x01mg | MyBiosource | 5x0.1mg | EUR 1885 |
Rabbit anti-Simian immunodeficiency virus AGM.tantalus (SIV-agm.tan)(Simian immunodeficiency virus African green monkey tantalus) VIF Polyclonal Antibody |
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MBS7149894-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) NEF Polyclonal Antibody |
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MBS7152364-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) VPR Polyclonal Antibody |
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MBS7152370-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) ENV Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) VIF Polyclonal Antibody |
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MBS7151705-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) VPX Polyclonal Antibody |
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MBS7151883-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) GAG Polyclonal Antibody |
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MBS7151995-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) TAT Polyclonal Antibody |
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MBS7151999-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) REV Polyclonal Antibody |
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MBS7152178-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate Mm142-83)(SIV-mac)(Simian immunodeficiency virus rhesus monkey) gag-pol Polyclonal Antibody |
|||
MBS7162165-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate F236/smH4)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) gag-pol Polyclonal Antibody |
|||
MBS7152367-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) REV Polyclonal Antibody |
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MBS7152555-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) TAT Polyclonal Antibody |
|||
MBS7152556-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) NEF Polyclonal Antibody |
|||
MBS7152785-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) GAG Polyclonal Antibody |
|||
MBS7152940-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) VPR Polyclonal Antibody |
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MBS7152941-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) VIF Polyclonal Antibody |
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MBS7153144-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) ENV Polyclonal Antibody |
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MBS7153629-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus (isolate PBj14/BCL-3)(SIV-sm)(Simian immunodeficiency virus sooty mangabey monkey) gag-pol Polyclonal Antibody |
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MBS7153371-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) GAG Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) NEF Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM266)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) POL Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM385)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) POL Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) ENV Polyclonal Antibody |
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MBS7154765-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) ENV Polyclonal Antibody |
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MBS7154773-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) VIF Polyclonal Antibody |
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Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) REV Polyclonal Antibody |
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MBS7155073-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) GAG Polyclonal Antibody |
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MBS7155074-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) VPX Polyclonal Antibody |
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MBS7155075-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) NEF Polyclonal Antibody |
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MBS7156175-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) VIF Polyclonal Antibody |
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MBS7156176-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) REV Polyclonal Antibody |
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MBS7156177-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) VPX Polyclonal Antibody |
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MBS7156968-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) TAT Polyclonal Antibody |
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MBS7158026-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) TAT Polyclonal Antibody |
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MBS7158841-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Recombinant Simian Immunodeficiency Virus |
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MBS141703-0002mg | MyBiosource | 0.002mg | EUR 240 |
Recombinant Simian Immunodeficiency Virus |
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MBS141703-001mg | MyBiosource | 0.01mg | EUR 310 |
Recombinant Simian Immunodeficiency Virus |
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MBS141703-01mg | MyBiosource | 0.1mg | EUR 1260 |
Recombinant Simian Immunodeficiency Virus |
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MBS141703-5x01mg | MyBiosource | 5x0.1mg | EUR 5345 |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM3)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) gag-pol Polyclonal Antibody |
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MBS7156969-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.vervet (isolate AGM155)(SIV-agm.ver)(Simian immunodeficiency virus African green monkey vervet) gag-pol Polyclonal Antibody |
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MBS7158840-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.grivet (isolate AGM gr-1)(SIV-agm.gri)(Simian immunodeficiency virus African green monkey grivet) ENV Polyclonal Antibody |
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MBS7150276-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.grivet (isolate AGM gr-1)(SIV-agm.gri)(Simian immunodeficiency virus African green monkey grivet) GAG Polyclonal Antibody |
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MBS7150277-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Rabbit anti-Simian immunodeficiency virus agm.grivet (isolate AGM gr-1)(SIV-agm.gri)(Simian immunodeficiency virus African green monkey grivet) VPX Polyclonal Antibody |
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MBS7144516-INQUIRE | MyBiosource | INQUIRE | Ask for price |
Design for preparation of extra lively cross-linked enzyme aggregates of Burkholderia cepacia lipase utilizing palm fiber residue
A brand new design of cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase (BCL) based mostly primarily on the usage of lignocellulosic residue of palm fiber as an additive was proposed. Totally different parameters for the preparation of lively CLEAs within the hydrolysis of olive oil, comparable to precipitation brokers, crosslinking agent focus, components, and coating brokers had been investigated. The very best exercise yield (121.1 ± 0.1%) and volumetric exercise (1578.1 ± 2.5 U/mL) had been achieved for CLEAs ready utilizing the mix of a coating step with Triton® X-100 and polyethyleneimine plus the usage of palm fiber as an additive.
The variations of the secondary constructions of BCL-CLEAs had been analyzed by second-derivative infrared spectra, primarily indicating a discount of the α-helix construction, which was chargeable for the lipase activation within the supramolecular construction of the CLEAs.
Thus, these outcomes supplied proof of an revolutionary design of BCL-CLEAs as a sustainable and biocompatible alternative for biotechnology purposes.
Key phrases: Burkholderia cepacia lipase; Cross-linked enzyme aggregates; Hydrolysis reactions; Palm fiber.
The Amino-Functionalized Graphene Oxide Nanosheet Preparation for Enzyme Covalent Immobilization
Enzyme immobilization has demonstrated efficient means for extending protein stability and shelf life. Nevertheless, present strategies negatively have an effect on the enzyme exercise, notably for utility functions.
Herein, the amino functionalized graphene oxide (GO-NH₂) was synthesized and used to covalently immobilize lactase. FT-IR, UV-Vis, SEM, TEM and XPS had been employed to verify and characterize the immobilized lactase. On the ensuing optimum temperature, the immobilization charge achieved 61% and the immobilized lactase maintained roughly 95% of catalyst exercise.
In contrast with the free lactase, used as a management, the immobilized lactase was considerably extra steady inside acidic or fundamental environments, greater temperature situations and had a number of recycle use traits.
Moreover, the immobilized lactase had a preserved 87% exercise after storage at 4 °C for 30 days, whereas the free lactase was virtually deactivated after 20 days underneath the identical storage situations. This work conforms that the amino-functionalized graphene oxide is a possible assist materials for lactase immobilization and will be prolonged to make use of with a wide range of enzymes for a variety of purposes.
Human IgM ELISA Kit |
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DEIA691 | Creative Diagnostics | 96T | EUR 752.4 |
Description: This Kit is a highly sensitive twosite enzyme linked immunoassay (ELISA) for measuring IgM in Human biological samples. |
Human (IgM) ELISA kit |
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E01A1252 | BlueGene | 96T | EUR 700 |
Description: ELISA |
Human IgM ELISA KIT |
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E42EH-154 | EnoGene | 96T/48T | Ask for price |
Human IgM ELISA kit |
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E22-HC019.48 | EnoGene | 48T | EUR 295 |
Human IgM ELISA kit |
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E22-HC019.96 | EnoGene | 96T | EUR 395 |
Human IgM ELISA Kit |
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E28L0255 | EnoGene | 96T | EUR 666.67 |
Human IgM ELISA Kit |
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E28L0255-48 | EnoGene | 48T | EUR 380.95 |
Human IgM ELISA Kit |
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E28L0255-96 | EnoGene | 96T | EUR 634.92 |
Human IgM ELISA kit |
|||
55R-1920 | Fitzgerald | 96 tests | EUR 508 |
Description: ELISA Kit for detection of IgM in the research laboratory |
Human IgM ELISA KIT |
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EF000297 | Nova Lifetech | 96tests | EUR 566 |
Human IgM ELISA Kit |
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ELA-E0543h | Nova Lifetech | 96tests | EUR 696 |
Human IgM ELISA kit |
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F170 | Cygnus Technologies | 1 kit (96 wells plate) | EUR 1056 |
Description: Human IgM ELISA kit by Cygnus Technologies is available in Europe via Gentaur. |
*Human IgM ELISA Kit |
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IHUIGMKT | Innovative research | each | EUR 347 |
Description: *Human IgM ELISA Kit |
Human IgM ELISA Kit |
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GWB-962048 | GenWay Biotech | 96 well | Ask for price |
Human IgM ELISA Kit |
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RK00097 | Abclonal | 96 Tests | EUR 280 |
Human IgM ELISA Kit |
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MBS8291366-5x96Tests | MyBiosource | 5x96Tests | EUR 2755 |
Human IgM ELISA Kit |
|||
MBS8291366-96Tests | MyBiosource | 96Tests | EUR 620 |
Human IgM ELISA Kit |
|||
MBS8579160-48Tests | MyBiosource | 48Tests | EUR 390 |
Human IgM ELISA Kit |
|||
MBS8579160-5x96Tests | MyBiosource | 5x96Tests | EUR 2275 |
Human IgM ELISA Kit |
|||
MBS8579160-96Tests | MyBiosource | 96Tests | EUR 495 |
Human IgM ELISA Kit |
|||
MBS9135808-5x96Tests | MyBiosource | 5x96Tests | EUR 2615 |
Human IgM ELISA Kit |
|||
MBS9135808-96Tests | MyBiosource | 96Tests | EUR 570 |
Human IgM ELISA Kit |
|||
MBS564051-5x96Wells | MyBiosource | 5x96Wells | EUR 1915 |
Human IgM ELISA Kit |
|||
MBS564051-96Wells | MyBiosource | 96Wells | EUR 420 |
Human Zonulin IgM (HP IgM) ELISA Kit |
|||
abx055946-100g | Abbexa | 100 µg | Ask for price |
Human Zonulin IgM (HP IgM) ELISA Kit |
|||
abx055946-10g | Abbexa | 10 µg | EUR 493.75 |
Human Zonulin IgM (HP IgM) ELISA Kit |
|||
abx055946-50g | Abbexa | 50 µg | Ask for price |
Human Rotavirus IgM (RV IgM) ELISA Kit |
|||
abx051596-96tests | Abbexa | 96 tests | EUR 801.6 |
Human Rotavirus IgM,RV IgM ELISA KIT |
|||
ED0232Hu | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human Rotavirus IgM (RV IgM) ELISA Kit |
|||
abx051596-100g | Abbexa | 100 µg | Ask for price |
Human Rotavirus IgM (RV IgM) ELISA Kit |
|||
abx051596-200g | Abbexa | 200 µg | Ask for price |
Human Rotavirus IgM (RV IgM) ELISA Kit |
|||
abx051596-50g | Abbexa | 50 µg | EUR 493.75 |
Human Rotavirus IgM, RV IgM ELISA Kit |
|||
MBS1610579-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Rotavirus IgM, RV IgM ELISA Kit |
|||
MBS1610579-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Rotavirus IgM, RV IgM ELISA Kit |
|||
MBS1610579-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Human Rotavirus IgM, RV IgM ELISA Kit |
|||
MBS167848-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3375 |
Human Rotavirus IgM, RV IgM ELISA Kit |
|||
MBS167848-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1715 |
Human Rotavirus IgM, RV IgM ELISA Kit |
|||
MBS167848-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Human Hantavirus IgM(HV- IgM) Elisa Kit |
|||
EK711721 | AFG Bioscience LLC | 96 Wells | EUR 0.2 |
Human Hantavirus IgM (HV IgM) ELISA Kit |
|||
abx055453-100g | Abbexa | 100 µg | Ask for price |
Human Hantavirus IgM (HV IgM) ELISA Kit |
|||
abx055453-10g | Abbexa | 10 µg | EUR 493.75 |
Human Hantavirus IgM (HV IgM) ELISA Kit |
|||
abx055453-50g | Abbexa | 50 µg | Ask for price |
Human Occludin IgM (OCLN IgM) ELISA Kit |
|||
abx055943-100g | Abbexa | 100 µg | Ask for price |
Human Occludin IgM (OCLN IgM) ELISA Kit |
|||
abx055943-10g | Abbexa | 10 µg | EUR 493.75 |
Human Occludin IgM (OCLN IgM) ELISA Kit |
|||
abx055943-50g | Abbexa | 50 µg | Ask for price |
Human Hantavirus IgM(HV- IgM) ELISA Kit |
|||
SL2206Hu | Sunlong | 96 Tests | EUR 468 |
Human EchoVirus IgM(ECHO IgM) Elisa Kit |
|||
EK700521 | AFG Bioscience LLC | 96 Wells | EUR 0.5 |
Human EchoVirus IgM(ECHO IgM)ELISA Kit |
|||
GA-E1779HM-48Tests | GenAsia Biotech | 48 Tests | Ask for price |
Human EchoVirus IgM(ECHO IgM)ELISA Kit |
|||
GA-E1779HM-96Tests | GenAsia Biotech | 96 Tests | EUR 388 |
Human Leptospira IgM,Lep IgM ELISA KIT |
|||
ED0087Hu | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human Enterovirus IgM,EV IgM ELISA KIT |
|||
ED0525Hu | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human EchoVirus IgM(ECHO IgM)ELISA Kit |
|||
GA-E1779HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human EchoVirus IgM(ECHO IgM)ELISA Kit |
|||
GA-E1779HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human EchoVirus IgM(ECHO IgM)ELISA Kit |
|||
QY-E04350 | Qayee Biotechnology | 96T | EUR 433.2 |
Human Leptospira IgM,Lep IgM ELISA Kit |
|||
SL2927Hu | Sunlong | 96 Tests | EUR 468 |
Human EchoVirus IgM,ECHO IgM ELISA Kit |
|||
SL0639Hu | Sunlong | 96 Tests | EUR 468 |
Human Adenovirus IgM (ADV IgM) ELISA Kit |
|||
abx052156-100g | Abbexa | 100 µg | Ask for price |
Human Adenovirus IgM (ADV IgM) ELISA Kit |
|||
abx052156-10g | Abbexa | 10 µg | EUR 493.75 |
Human Adenovirus IgM (ADV IgM) ELISA Kit |
|||
abx052156-50g | Abbexa | 50 µg | Ask for price |
Human Adenovirus IgM (ADV IgM) ELISA Kit |
|||
abx364855-100g | Abbexa | 100 µg | EUR 493.75 |
Human Echovirus IgM (ECHO IgM) ELISA Kit |
|||
abx364937-100g | Abbexa | 100 µg | EUR 493.75 |
human EchoVirus IgM, ECHO IgM ELISA Kit |
|||
MBS703446-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 5325 |
human EchoVirus IgM, ECHO IgM ELISA Kit |
|||
MBS703446-24StripWellsLIMIT1 | MyBiosource | 24-Strip-Wells(LIMIT1) | EUR 275 |
human EchoVirus IgM, ECHO IgM ELISA Kit |
|||
MBS703446-48StripWells | MyBiosource | 48-Strip-Wells | EUR 480 |
human EchoVirus IgM, ECHO IgM ELISA Kit |
|||
MBS703446-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 2795 |
human EchoVirus IgM, ECHO IgM ELISA Kit |
|||
MBS703446-96StripWells | MyBiosource | 96-Strip-Wells | EUR 675 |
Human Leptospira IgM, Lep IgM ELISA Kit |
|||
MBS1610473-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Leptospira IgM, Lep IgM ELISA Kit |
|||
MBS1610473-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Leptospira IgM, Lep IgM ELISA Kit |
|||
MBS1610473-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
Human Enterovirus IgM, EV IgM ELISA Kit |
|||
MBS1610787-10x96StripWells | MyBiosource | 10x96-Strip-Wells | EUR 3460 |
Human Enterovirus IgM, EV IgM ELISA Kit |
|||
MBS1610787-5x96StripWells | MyBiosource | 5x96-Strip-Wells | EUR 1750 |
Human Enterovirus IgM, EV IgM ELISA Kit |
|||
MBS1610787-96StripWells | MyBiosource | 96-Strip-Wells | EUR 425 |
human EchoVirus IgM,ECHO IgM ELISA Kit |
|||
201-12-1763 | Shanghai Sunred Biological Technology | 96 tests | EUR 528 |
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids. |
Human measlesvirus IgM(MV IgM)ELISA Kit |
|||
GA-E1781HM-48Tests | GenAsia Biotech | 48 Tests | Ask for price |
Human measlesvirus IgM(MV IgM)ELISA Kit |
|||
GA-E1781HM-96Tests | GenAsia Biotech | 96 Tests | EUR 388 |
Human measlesvirus IgM,MV IgM ELISA KIT |
|||
ED0125Hu | Jiaxing Korain Biotech Ltd (BT Labs) | 96 wells | EUR 458 |
Human measlesvirus IgM(MV IgM)ELISA Kit |
|||
GA-E1781HM-48T | GenAsia Biotech | 48T | EUR 346.8 |
Human measlesvirus IgM(MV IgM)ELISA Kit |
|||
GA-E1781HM-96T | GenAsia Biotech | 96T | EUR 559.2 |
Human measlesvirus IgM(MV IgM)ELISA Kit |
|||
QY-E02205 | Qayee Biotechnology | 96T | EUR 433.2 |
Human measlesvirus IgM,MV IgM ELISA Kit |
|||
SL1162Hu | Sunlong | 96 Tests | EUR 468 |