PCR merchandise for electrospray ionization mass spectrometry utilizing the DNA

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Preparation of single-stranded PCR merchandise for electrospray ionization mass spectrometry utilizing the DNA restore enzyme lambda exonuclease.

Electrospray ionization mass spectrometry (ESI-MS) has been utilized to acquire correct mass measurements of intact PCR merchandise; nonetheless, single-stranded PCR merchandise are essential to detect sequence modifications comparable to base substitutions, additions or deletions. The areas of those modifications can subsequently be decided utilizing extra phases of mass spectrometry.
  • The recombinant enzyme lambda exonuclease selectively digests one strand of a DNA duplex from a 5′ phosphorylated finish leaving the complementary strand intact. Utilizing this speedy enzymatic step, we had been capable of produce single-stranded PCR merchandise by digestion of an intact PCR product derived from the Human Tyrosine Hydroxylase (HUMTHO1) gene, which incorporates a tetrameric repeating motif.
  • The non-template directed 3′ adenylation frequent when utilizing Taq polymerase resulted in three distinct species (blunt-ended, mono-adenylated and di-adenylated), which added complexity to the spectrum of the double-stranded product.
  • The info from the single-stranded merchandise reveals that one strand is preferentially adenylated over the opposite, which can’t be decided from the mass spectrum of the double-stranded PCR product alone. The ESI-FTICR (Fourier rework ion cyclotron resonance) mass spectra of the lambda exonuclease handled PCR merchandise exhibited lower than anticipated signal-to-noise (S/N) ratios.
  • That is attributed to inaccurate focus calculations attributable to remaining double-stranded PCR product amplified with unphosphorylated primers, and to matrix results contributed by the lambda exonuclease response buffer.
  • To additional take a look at this speculation, we investigated and decided the limit of detection to be 0.27 microM utilizing customary curve statistics for single acquisitions of an artificial 75-mer. The concentrations of the noncoding and coding strands produced by lambda exonuclease digestion had been calculated to be 0.29 and 0.37 microM, respectively, bearing in mind the presence of double-stranded product.
  • The merchandise had been electrosprayed from concentrations on the restrict of detection requiring the averaging of 5-10 acquisitions to provide a enough S/N ratio, indicating that product focus, base composition and matrix results play a mixed, vital position in detection of lambda exonuclease handled PCR merchandise.
  • Though extra work shall be required to additional exploit this technique, lambda exonuclease clearly gives mass spectrometrists with a technique to generate single-stranded PCR merchandise.

 

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Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

CSB-EP323941SHD 2877 mg Ask for price

Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

CSB-EP320847SHA 2814 mg Ask for price

Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

RPC22470-100ug 100ug
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Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

RPC22470-1mg 1mg
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Recombinant Simian immunodeficiency virus Protein Vpx (vpx)

RPC22470-20ug 20ug
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Feline Immunodeficiency Virus (FIV) Antigen

abx160063-100g 100 µg Ask for price

Feline Immunodeficiency Virus (FIV) Antigen

abx160063-10g 10 µg
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Feline Immunodeficiency Virus (FIV) Antigen

abx160063-50g 50 µg Ask for price

Mouse Anti Human Immunodeficiency Virus (HIV-1) p17 (1981)

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Mouse Anti Human Immunodeficiency Virus (HIV-1) p17 (1981)

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REC31743-100 0.1
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Human Immunodeficiency Virus p24 Protein [HIV-1/Clade B]

REC31743-500 0.5
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Mouse Anti Human Immunodeficiency Virus (HIV-1) gp41 (1911)

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Description: Mouse monoclonal antibody specific for HIV-1 gp41

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Mouse Anti Human Immunodeficiency Virus (HIV-1) Integrase (2E3)

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Bovine immunodeficiency virus

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Bovine immunodeficiency virus

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Feline Immunodeficiency Virus p24 Antibody

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OPEF01524-1MG 1mg
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Feline Immunodeficiency Virus Antibody (p24 gag)

GWB-AA7524 0.25 mg Ask for price

Feline Immunodeficiency Virus Antibody (p24 gag)

GWB-ABCB24 0.25 mg Ask for price

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Feline immunodeficiency virus PCR kit

PCR-V175-48R 50T
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GWB-86DFD2 0.25 mg Ask for price

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GWB-30B103 0.25 mg Ask for price

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GWB-B4D6F2 0.2 mg Ask for price

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GWB-E938E9 0.25 mg Ask for price

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GWB-C4000D 2 ml Ask for price

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RTq-V234-100R 100T
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Bovine immunodeficiency virus RT PCR kit

RTq-V234-50R 50T
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Feline immunodeficiency virus RT PCR kit

RTq-V175-100R 100T
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RTq-V175-150R 150T
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HUMAN IMMUNODEFICIENCY VIRUS 1 gp41 (ECTO)

GWB-E0A020 0.1 mg Ask for price

Feline Immunodeficiency Virus Envelope Antibody

GWB-AD4CD5 0.2 mg Ask for price

Human immunodeficiency virus-Tat Protein (HIV-Tat) ELISA

201-12-1915 96 tests
EUR 528
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Human immunodeficiency virus-Tat Protein(HIV-Tat)ELISA

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Human immunodeficiency virus-Tat Protein(HIV-Tat)ELISA

QY-E02393 96T
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Design for preparation of extra lively cross-linked enzyme aggregates of Burkholderia cepacia lipase utilizing palm fiber residue

A brand new design of cross-linked enzyme aggregates (CLEAs) of Burkholderia cepacia lipase (BCL) based mostly primarily on the usage of lignocellulosic residue of palm fiber as an additive was proposed. Totally different parameters for the preparation of lively CLEAs within the hydrolysis of olive oil, comparable to precipitation brokers, crosslinking agent focus, components, and coating brokers had been investigated. The very best exercise yield (121.1 ± 0.1%) and volumetric exercise (1578.1 ± 2.5 U/mL) had been achieved for CLEAs ready utilizing the mix of a coating step with Triton® X-100 and polyethyleneimine plus the usage of palm fiber as an additive.
The variations of the secondary constructions of BCL-CLEAs had been analyzed by second-derivative infrared spectra, primarily indicating a discount of the α-helix construction, which was chargeable for the lipase activation within the supramolecular construction of the CLEAs.

Thus, these outcomes supplied proof of an revolutionary design of BCL-CLEAs as a sustainable and biocompatible alternative for biotechnology purposes.

Key phrases: Burkholderia cepacia lipase; Cross-linked enzyme aggregates; Hydrolysis reactions; Palm fiber.

The Amino-Functionalized Graphene Oxide Nanosheet Preparation for Enzyme Covalent Immobilization

Enzyme immobilization has demonstrated efficient means for extending protein stability and shelf life. Nevertheless, present strategies negatively have an effect on the enzyme exercise, notably for utility functions.
Herein, the amino functionalized graphene oxide (GO-NH₂) was synthesized and used to covalently immobilize lactase. FT-IR, UV-Vis, SEM, TEM and XPS had been employed to verify and characterize the immobilized lactase. On the ensuing optimum temperature, the immobilization charge achieved 61% and the immobilized lactase maintained roughly 95% of catalyst exercise.
In contrast with the free lactase, used as a management, the immobilized lactase was considerably extra steady inside acidic or fundamental environments, greater temperature situations and had a number of recycle use traits.
Moreover, the immobilized lactase had a preserved 87% exercise after storage at 4 °C for 30 days, whereas the free lactase was virtually deactivated after 20 days underneath the identical storage situations. This work conforms that the amino-functionalized graphene oxide is a possible assist materials for lactase immobilization and will be prolonged to make use of with a wide range of enzymes for a variety of purposes.

Human IgM ELISA Kit

E16HE0096 96T
EUR 833.33

Human IgM ELISA Kit

EHI0045 96Tests
EUR 625.2

Human IgM ELISA Kit

EHI0104 96Tests
EUR 625.2

Human IgM ELISA Kit

ELA-E0543h 96 Tests
EUR 988.8

Human IgM ELISA Kit

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EUR 752.4
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Human (IgM) ELISA kit

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E42EH-154 96T/48T Ask for price

Human IgM ELISA kit

E22-HC019.48 48T
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Human IgM ELISA kit

E22-HC019.96 96T
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Human IgM ELISA Kit

E28L0255 96T
EUR 666.67

Human IgM ELISA Kit

E28L0255-48 48T
EUR 380.95

Human IgM ELISA Kit

E28L0255-96 96T
EUR 634.92

Human IgM ELISA kit

55R-1920 96 tests
EUR 508
Description: ELISA Kit for detection of IgM in the research laboratory

*Human IgM ELISA Kit

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EUR 347
Description: *Human IgM ELISA Kit

Human IgM ELISA Kit

GWB-962048 96 well Ask for price

Human IgM ELISA kit

F170 1 kit (96 wells plate)
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Human IgM ELISA Kit

RK00097 96 Tests
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Human Rotavirus IgM (RV IgM) ELISA Kit

abx051596-96tests 96 tests
EUR 801.6

Human Rotavirus IgM (RV IgM) ELISA Kit

abx051596-100g 100 µg Ask for price

Human Rotavirus IgM (RV IgM) ELISA Kit

abx051596-200g 200 µg Ask for price

Human Rotavirus IgM (RV IgM) ELISA Kit

abx051596-50g 50 µg
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Human Hantavirus IgM(HV- IgM) Elisa Kit

EK711721 96 Wells
EUR 0.2

Human Hantavirus IgM(HV- IgM) ELISA Kit

SL2206Hu 96 Tests
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Human Hantavirus IgM (HV IgM) ELISA Kit

abx055453-100g 100 µg Ask for price

Human Hantavirus IgM (HV IgM) ELISA Kit

abx055453-10g 10 µg
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Human Hantavirus IgM (HV IgM) ELISA Kit

abx055453-50g 50 µg Ask for price

Human Occludin IgM (OCLN IgM) ELISA Kit

abx055943-100g 100 µg Ask for price

Human Occludin IgM (OCLN IgM) ELISA Kit

abx055943-10g 10 µg
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Human Occludin IgM (OCLN IgM) ELISA Kit

abx055943-50g 50 µg Ask for price

human EchoVirus IgM,ECHO IgM ELISA Kit

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Human EchoVirus IgM(ECHO IgM) Elisa Kit

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EUR 0.5

Human EchoVirus IgM(ECHO IgM)ELISA Kit

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Human EchoVirus IgM(ECHO IgM)ELISA Kit

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Human EchoVirus IgM(ECHO IgM)ELISA Kit

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Human Leptospira IgM,Lep IgM ELISA Kit

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Human Adenovirus IgM (ADV IgM) ELISA Kit

abx052156-100g 100 µg Ask for price

Human Adenovirus IgM (ADV IgM) ELISA Kit

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Human Adenovirus IgM (ADV IgM) ELISA Kit

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Human Adenovirus IgM (ADV IgM) ELISA Kit

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Human measlesvirus IgM(MV IgM)ELISA Kit

GA-E1781HM-48T 48T
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Human measlesvirus IgM(MV IgM)ELISA Kit

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Human measlesvirus IgM(MV IgM)ELISA Kit

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Human measlesvirus IgM,MV IgM ELISA Kit

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Human Papillomavirus IgM (HPV IgM) ELISA Kit

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Human Papillomavirus IgM (HPV IgM) ELISA Kit

abx052751-100g 100 µg Ask for price

Human Papillomavirus IgM (HPV IgM) ELISA Kit

abx052751-10g 10 µg
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Human Papillomavirus IgM (HPV IgM) ELISA Kit

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Human Cytomegalovirus IgM (CMV IgM) ELISA Kit

abx054250-100g 100 µg Ask for price

Human Cytomegalovirus IgM (CMV IgM) ELISA Kit

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Human Cytomegalovirus IgM (CMV IgM) ELISA Kit

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Human Lipopolysaccharides IgM (LPS IgM) ELISA Kit

abx055948-100g 100 µg Ask for price

Human Lipopolysaccharides IgM (LPS IgM) ELISA Kit

abx055948-10g 10 µg
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Human Lipopolysaccharides IgM (LPS IgM) ELISA Kit

abx055948-50g 50 µg Ask for price

Human Mumps Virus IgM (MuV IgM) ELISA Kit

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Human Zika Virus IgM (ZIKV IgM) ELISA Kit

abx055830-100g 100 µg Ask for price

Human Zika Virus IgM (ZIKV IgM) ELISA Kit

abx055830-10g 10 µg
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Human Zika Virus IgM (ZIKV IgM) ELISA Kit

abx055830-50g 50 µg Ask for price

Human Mumps Virus IgM (MuV IgM) ELISA Kit

abx364942-100g 100 µg
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Human Measles Virus IgM (MV IgM) ELISA Kit

abx364863-96tests 96 tests
EUR 651.6

Human Rubella Virus IgM (RV IgM) ELISA Kit

abx054089-100g 100 µg Ask for price

Human Rubella Virus IgM (RV IgM) ELISA Kit

abx054089-10g 10 µg
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Human Rubella Virus IgM (RV IgM) ELISA Kit

abx054089-50g 50 µg Ask for price

Human Measles Virus IgM (MV IgM) ELISA Kit

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Human Coronaviruses IgM (Coronaviruses- IgM)ELISA Kit

SL3089Hu 96 Tests
EUR 468

Human Adenovirus (ADV) IgM (ADV IgM) ELISA Kit

abx364855-96tests 96 tests
EUR 613.2

Human Enterovirus 71 IgM (EV71 IgM) ELISA Kit

abx364974-100g 100 µg
EUR 493.75

Human Parvovirus B19 IgM (B19V IgM) ELISA Kit

abx365089-100g 100 µg
EUR 618.75

Human EB IgM ELISA Kit

EHE0014 96Tests
EUR 625.2

Human IM IgM ELISA Kit

EHI0009 96Tests
EUR 625.2

Human RF IgM ELISA Kit

DEIA1697 96T
EUR 836.4
Description: The Rheumatoid Factor (RF) IgM ELISA test system is intended for the qualitative and semi-quantitative detection of RF IgM-class antibody in human serum. The test system is intended to be used as an aid in the diagnosis of rheumatoid arthritis.

Human RF IgM ELISA Kit

DEIA1810 96T
EUR 836.4
Description: Rheumatoid Factor IgM is an indirect solid phase enzyme immunoassay ( ELISA ) for the quantitative measurement of IgM class rheumatoid factor antibodies in human serum or plasma. The assay is intended for in vitro diagnostic use only as an aid in the determination of rheumatoid arthritis ( RA ).

Human MP IgM ELISA Kit

EHM0034 96Tests
EUR 625.2

Human Fasciola Hepatica IgM(FH IgM)ELISA Kit

YLA4252HU-48T 48T Ask for price

Human Fasciola Hepatica IgM(FH IgM)ELISA Kit

YLA4252HU-96T 96T Ask for price

Human Monkeypox Virus IgM (MPXV IgM) ELISA Kit

abx392299-100g 100 µg
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Human Rotavirus antibody IgM(RV IgM) Elisa Kit

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EUR 0.17

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