Automated, high-throughput applied sciences have gotten more and more frequent in microbiome research to lower prices and improve effectivity. Nevertheless, in microbiome research, small variations in methodology – together with storage situations, moist lab strategies, sequencing platforms and knowledge evaluation – can affect the reproducibility and comparability of knowledge throughout research. There was restricted testing of the results of high-throughput strategies, together with microfluidic PCR applied sciences.
On this paper, we evaluate two extraction strategies (the QIAamp DNA Stool Mini Equipment and the MoBio PowerSoil DNA Isolation package), two taq polymerase enzymes (MyTaq HS Purple Combine and Accustart II PCR ToughMix), two primer units (V3-V4 and V4-V5) and two amplification strategies (a typical two-step PCR protocol and amplicon library preparation on the Fluidigm Entry Array system that permits automated multiplexing of primers). Intestine microbial group profiles had been considerably affected by all variables. Whereas there have been no vital variations in alpha range measured between the 2 extraction strategies, there was an impact of extraction technique on group composition measured by unweighted UniFrac distances. Each amplification technique and primers had a big impact on each alpha range and group composition.
The relative abundance of Actinobacteria was considerably decrease when utilizing the MoBio package or Fluidigm amplification technique, and the relative abundance of Firmicutes was decrease when utilizing the Qiagen package. Microbial group profiles based mostly on Fluidigm-generated amplicon libraries weren’t corresponding to these generated with extra generally used strategies. Researchers ought to fastidiously contemplate the constraints and biases that completely different extraction and amplification strategies can introduce into their outcomes. Moreover, extra thorough benchmarking of automated and multiplexing strategies is critical to find out the magnitude of the potential trade-off between the standard and the amount of knowledge.
Diagnostic approaches in COVID-19: medical updates
[Detoxification mechanism of Aconiti Lateralis Radix Praeparata combined with dried Rehmanniae Radix based on metabolic enzymes in liver]
Coelenterazine CP |
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10112-2 | Biotium | 250uG | EUR 257 |
Description: N/A |
Coelenterazine CP |
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10112-2-1 | Biotium | EA | EUR 257 |
CP-547632 |
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200810 | MedKoo Biosciences | 5.0mg | EUR 300 |
CP-724714 |
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200820 | MedKoo Biosciences | 5.0mg | EUR 250 |
CP-690550 |
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1622-25 | Biovision | each | EUR 738 |
CP-690550 |
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1622-5 | Biovision | each | EUR 255.6 |
CP-609754 |
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206096 | MedKoo Biosciences | 10.0mg | EUR 450 |
CP 80633 |
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27301 | BPS Bioscience | 10 mg | EUR 310 |
Description: CP 80633 is a selective inhibitor of PDE4, which does not display significant isozyme selectivity. It also inhibits hydrolysis of cAMP in isolated monocytes, eosinophils, human peripheral blood and T-cells. It has been shown to exhibit anti-inflammatory and bronchodilatory activity in vivo. |
CP 135807 |
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413313 | MedKoo Biosciences | 5.0mg | EUR 275 |
CP-346086 |
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471083 | MedKoo Biosciences | 5.0mg | EUR 270 |
CP-335963 |
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471085 | MedKoo Biosciences | 1.0g | EUR 170 |
CP-101537 |
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471091 | MedKoo Biosciences | 10.0mg | EUR 90 |
CP Antibody |
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E046543 | EnoGene | 100μg/100μl | EUR 255 |
Description: Available in various conjugation types. |
CP Antibody |
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E19-9369-1 | EnoGene | 50ug/50ul | EUR 145 |
Description: Available in various conjugation types. |
CP Antibody |
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E19-9369-2 | EnoGene | 100ug/100ul | EUR 225 |
Description: Available in various conjugation types. |
CP Antibody |
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F50324-0.4ML | NSJ Bioreagents | 0.4 ml | EUR 322.15 |
Description: Ceruloplasmin is a metalloprotein that binds most of the copper in plasma and is involved in the peroxidation of Fe(II) transferrin to Fe(III) transferrin. Mutations in this protein cause aceruloplasminemia, which results in iron accumulation and tissue damage, and is associated with diabetes and neurologic abnormalities. |
CP-724714 |
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GW3270-1 | Glentham Life Sciences | 1 | EUR 171.5 |
CP-724714 |
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GW3270-10 | Glentham Life Sciences | 10 | EUR 401.4 |
CP-724714 |
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GW3270-5 | Glentham Life Sciences | 5 | EUR 254 |
CP Antibody |
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DF9369 | Affbiotech | 200ul | EUR 420 |
CP Antibody |
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DF9369-100ul | Affinity Biosciences | 100ul | EUR 280 |
CP Antibody |
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DF9369-200ul | Affinity Biosciences | 200ul | EUR 350 |
CP siRNA |
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20-abx912716 | Abbexa |
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CP siRNA |
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20-abx912717 | Abbexa |
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CP Antibody |
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46543 | SAB | 100ul | EUR 319 |
CP Antibody |
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46543-100ul | SAB | 100ul | EUR 302.4 |
CP Antibody |
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45875-100ul | SAB | 100ul | EUR 302.4 |
CP Antibody |
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45875-50ul | SAB | 50ul | EUR 224.4 |
CP-809101 |
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A3330-10 | ApexBio | 10 mg | EUR 138 |
Description: 5-HT2C receptor agonist |
CP-809101 |
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A3330-50 | ApexBio | 50 mg | EUR 591 |
Description: 5-HT2C receptor agonist |
CP 471474 |
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A4435-10 | ApexBio | 10 mg | EUR 201 |
Description: Broad spectrum MMP inhibitor |
CP 471474 |
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A4435-50 | ApexBio | 50 mg | EUR 842 |
Description: Broad spectrum MMP inhibitor |
CP 43 |
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584471 | MedKoo Biosciences | 10.0mg | EUR 360 |
CP-380736 |
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561663 | MedKoo Biosciences | 5.0mg | EUR 255 |
CP-96345 |
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562171 | MedKoo Biosciences | 5.0mg | Ask for price |
CP-424174 |
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530552 | MedKoo Biosciences | 10.0mg | EUR 300 |
CP-74006 |
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530978 | MedKoo Biosciences | 500.0mg | EUR 350 |
CP-775146 |
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531747 | MedKoo Biosciences | 5.0mg | EUR 250 |
CP-226269 |
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513641 | MedKoo Biosciences | 5.0mg | EUR 275 |
CP-105696 |
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524768 | MedKoo Biosciences | 5.0mg | EUR 265 |
CP-55940 |
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524805 | MedKoo Biosciences | 10.0mg | EUR 600 |
CP 66713 |
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524810 | MedKoo Biosciences | 5.0mg | EUR 280 |
CP-471474 |
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524847 | MedKoo Biosciences | 10.0mg | EUR 295 |
CP-532623 |
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524851 | MedKoo Biosciences | 5.0mg | EUR 300 |
CP-673451 |
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524866 | MedKoo Biosciences | 5.0mg | EUR 350 |
CP-868388 |
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524879 | MedKoo Biosciences | 5.0mg | EUR 320 |
CP-91149 |
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524881 | MedKoo Biosciences | 10.0mg | EUR 240 |
CP-281384 |
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525432 | MedKoo Biosciences | 5.0mg | EUR 270 |
CP-944629 |
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526348 | MedKoo Biosciences | 5.0mg | EUR 270 |
CP-724714 |
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A2412-100 | ApexBio | 100 mg | EUR 589 |
Description: HER2 inhibitor,potent and selective |
CP-724714 |
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A2412-25 | ApexBio | 25 mg | EUR 235 |
Description: HER2 inhibitor,potent and selective |
CP-724714 |
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A2412-5 | ApexBio | 5 mg | EUR 68 |
Description: HER2 inhibitor,potent and selective |
CP-724714 |
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A2412-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 74 |
Description: HER2 inhibitor,potent and selective |
CP 316819 |
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B5448-10 | ApexBio | 10 mg | EUR 298 |
Description: Selective glycogen phosphorylase inhibitor |
CP 316819 |
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B5448-50 | ApexBio | 50 mg | EUR 1185 |
Description: Selective glycogen phosphorylase inhibitor |
CP 20961 |
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B5474-10 | ApexBio | 10 mg | EUR 226 |
Description: non-immunogenic adjuvant |
CP 20961 |
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B5474-50 | ApexBio | 50 mg | EUR 952 |
Description: non-immunogenic adjuvant |
CP 135807 |
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B7497-10 | ApexBio | 10 mg | EUR 270 |
Description: 5-HT1D receptor agonist |
CP 135807 |
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B7497-50 | ApexBio | 50 mg | EUR 1096 |
Description: 5-HT1D receptor agonist |
CP 775146 |
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B7609-10 | ApexBio | 10 mg | EUR 308 |
Description: PPARα agonist |
CP 775146 |
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B7609-50 | ApexBio | 50 mg | EUR 1234 |
Description: PPARα agonist |
CP-466722 |
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B1722-1 | Biovision | each | EUR 183.6 |
CP-466722 |
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B1722-5 | Biovision | each | EUR 418.8 |
CP-547632 |
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B2056-500 | Biovision | each | EUR 183.6 |
CP 80633 |
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B7162-10 | ApexBio | 10 mg | EUR 258 |
Description: PDE4 inhibitor |
CP 80633 |
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B7162-50 | ApexBio | 50 mg | EUR 1019 |
Description: PDE4 inhibitor |
CP 154526 |
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B7185-1 | ApexBio | 1 mg | EUR 48 |
Description: CRF1 receptor antagonist |
CP 154526 |
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B7185-5 | ApexBio | 5 mg | EUR 78 |
Description: CRF1 receptor antagonist |
CP 96345 |
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B7213-10 | ApexBio | 10 mg | EUR 285 |
Description: NK1 receptor antagonist |
CP 96345 |
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B7213-50 | ApexBio | 50 mg | EUR 1163 |
Description: NK1 receptor antagonist |
CP-640186 |
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B3585-10 | ApexBio | 10 mg | EUR 156 |
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-640186 |
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B3585-2 | ApexBio | 2 mg | EUR 64 |
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-640186 |
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B3585-5 | ApexBio | 5 mg | EUR 96 |
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-640186 |
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B3585-50 | ApexBio | 50 mg | EUR 616 |
Description: isozyme-nonselective acetyl-CoA carboxylase (ACCase) inhibitor |
CP-424174 |
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B2531-1 | Biovision | each | EUR 157.2 |
CP-424174 |
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B2531-5 | Biovision | each | EUR 405.6 |
CP-544326 |
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B1121-25 | Biovision | each | EUR 666 |
CP-544326 |
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B1121-5 | Biovision | each | EUR 222 |
CP-724714 |
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B1333-25 | Biovision | each | EUR 940.8 |
CP-724714 |
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B1333-5 | Biovision | each | EUR 314.4 |
CP-673451 |
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B1337-1 | Biovision | each | EUR 288 |
CP-673451 |
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B1337-5 | Biovision | each | EUR 810 |
CP-673451 |
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B2173-10 | ApexBio | 10 mg | EUR 180 |
Description: PDGFRα/β inhibitor,potent and selective |
CP-673451 |
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B2173-200 | ApexBio | 200 mg | EUR 1672 |
Description: PDGFRα/β inhibitor,potent and selective |
CP-673451 |
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B2173-5 | ApexBio | 5 mg | EUR 95 |
Description: PDGFRα/β inhibitor,potent and selective |
CP-673451 |
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B2173-5.1 | ApexBio | 10 mM (in 1mL DMSO) | EUR 128 |
Description: PDGFRα/β inhibitor,potent and selective |
CP-673451 |
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B2173-50 | ApexBio | 50 mg | EUR 543 |
Description: PDGFRα/β inhibitor,potent and selective |
CP Antibody |
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ABD9369 | Lifescience Market | 100 ug | EUR 525.6 |
CP-466722 |
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A8625-10 | ApexBio | 10 mg | EUR 136 |
Description: ATM inhibitor,potent and reversible |
CP-466722 |
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A8625-5 | ApexBio | 5 mg | EUR 88 |
Description: ATM inhibitor,potent and reversible |
CP-466722 |
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A8625-50 | ApexBio | 50 mg | EUR 374 |
Description: ATM inhibitor,potent and reversible |
CP-10447 |
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C781200 | Toronto Research Chemicals | 10mg | EUR 201 |
Description: 843-93-6 |
CP 96345 |
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C781210 | Toronto Research Chemicals | 250mg | EUR 9200 |
Description: 132746-60-2 |
(±)-CP 55940 |
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C781225 | Toronto Research Chemicals | 0.5mg | EUR 1518 |
CP-141938 |
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C781290 | Toronto Research Chemicals | 100mg | EUR 12800 |
Description: 182822-62-4 |
CP-316819 |
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C781300 | Toronto Research Chemicals | 10mg | EUR 356 |
CP-471474 |
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C781305 | Toronto Research Chemicals | 5mg | EUR 86 |
Description: 210755-45-6 |
CP-47497 |
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C781310 | Toronto Research Chemicals | 10mg | EUR 69 |
(-)-CP-47947 |
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C781325 | Toronto Research Chemicals | 25mg | EUR 31000 |
Description: 114753-51-4 |
CP-775146 |
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C781355 | Toronto Research Chemicals | 10mg | EUR 230 |
Description: 702680-17-9 |
CP-91149 |
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C781360 | Toronto Research Chemicals | 100mg | EUR 76 |
Description: 186392-40-5 |
CP 724714 |
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C781365 | Toronto Research Chemicals | 250mg | EUR 4500 |
Description: 383432-38-0 |
CP-466722 |
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C781395 | Toronto Research Chemicals | 10mg | EUR 1200 |
Description: 1080622-86-1 |
CP-481715 |
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C781405 | Toronto Research Chemicals | 100mg | EUR 9200 |
Description: 212790-31-3 |
(+)-CP 55940 |
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C781415 | Toronto Research Chemicals | 25mg | EUR 27000 |
Description: 83002-05-5 |
CP 640186 |
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C591780 | Toronto Research Chemicals | 100mg | EUR 4500 |
Description: 591778-68-6 |
CP Antibody |
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CAC09179-100ug | Biomatik Corporation | 100ug | EUR 314 |
CP Antibody |
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CAC09179-50ug | Biomatik Corporation | 50ug | EUR 199.2 |
CP Antibody |
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1-CSB-PA14599A0Rb | Cusabio |
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Description: A polyclonal antibody against CP. Recognizes CP from Human. This antibody is Unconjugated. Tested in the following application: ELISA, IHC; Recommended dilution: IHC:1:20-1:200 |
CP-316819 |
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574642 | MedKoo Biosciences | 10.0mg | EUR 330 |
CP-640186 |
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9422-25 | Biovision | each | EUR 666 |
CP-640186 |
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9422-5 | Biovision | each | EUR 222 |
CP-91149 |
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A8403-10 | ApexBio | 10 mg | EUR 105 |
Description: Selective inhibitor of glycogen phosphorylase |
CP-91149 |
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A8403-25 | ApexBio | 25 mg | EUR 210 |
Description: Selective inhibitor of glycogen phosphorylase |
CP-91149 |
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A8403-5 | ApexBio | 5 mg | EUR 63 |
Description: Selective inhibitor of glycogen phosphorylase |
CP 154,526 |
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T27057-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP 154,526 |
CP 154,526 |
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T27057-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP 154,526 |
CP 154,526 |
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T27057-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP 154,526 |
CP 154,526 |
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T27057-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: CP 154,526 |
CP 154,526 |
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T27057-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: CP 154,526 |
CP-122288 |
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T27059-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP-122288 |
CP-122288 |
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T27059-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP-122288 |
CP-122288 |
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T27059-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP-122288 |
CP-122288 |
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T27059-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: CP-122288 |
CP-122288 |
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T27059-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: CP-122288 |
CP-226269 |
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T27060-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP-226269 |
CP-226269 |
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T27060-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP-226269 |
CP-226269 |
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T27060-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP-226269 |
CP-226269 |
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T27060-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: CP-226269 |
CP-226269 |
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T27060-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: CP-226269 |
CP-281384 |
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T27062-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP-281384 |
CP-281384 |
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T27062-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP-281384 |
CP-281384 |
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T27062-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP-281384 |
CP-281384 |
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T27062-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: CP-281384 |
CP-281384 |
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T27062-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: CP-281384 |
CP-293019 |
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T27063-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP-293019 |
CP-293019 |
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T27063-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP-293019 |
CP-293019 |
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T27063-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP-293019 |
CP-293019 |
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T27063-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: CP-293019 |
CP-293019 |
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T27063-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: CP-293019 |
CP-312 |
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T27064-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP-312 |
CP-312 |
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T27064-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP-312 |
CP-312 |
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T27064-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP-312 |
CP-312 |
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T27064-50mg | TargetMol Chemicals | 50mg | Ask for price |
Description: CP-312 |
CP-312 |
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T27064-5mg | TargetMol Chemicals | 5mg | Ask for price |
Description: CP-312 |
CP-944629 |
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T27067-10mg | TargetMol Chemicals | 10mg | Ask for price |
Description: CP-944629 |
CP-944629 |
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T27067-1g | TargetMol Chemicals | 1g | Ask for price |
Description: CP-944629 |
CP-944629 |
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T27067-1mg | TargetMol Chemicals | 1mg | Ask for price |
Description: CP-944629 |